1. Isolation of Virus
2. Demonstrating four fold or greater rise in antibody titer in paired sera
3. Enzyme–Linked ImmunoSorbent Assay (ELISA) testing for IgM antibody.
4. Diagnosis by Real Time – Polymerase chain reaction (RT–PCR) Test

The IgM antibody will be positive after 5th day of illness only.

Treatment of Chikungunya Fever

• Chikungunya is a Viral Disease, therefore rest is important. Vigorous exercise should be avoided. Mild exercise is recommended.
  
  
• Plenty of fluids should be taken.
  
  
• Antipyretics & analgesics such as Paracetamol or Ibuprofen as per the recommendation of doctor should be taken.
  
  
• Avoid Aspirin or steroids in any form.
  
  
• There is no need of antibiotics or Intra Venous fluids like normal saline, dextrose routinely. If any associated conditions are there, then only these are necessary.

Incubation Period of Chikungunya Fever
It is usually 2 to 12 days. (Commonly 3 to 5 days)